By Barbara J. Bain
Blood Cells has been written with either the training haematologist and the trainee in brain. It goals to supply a consultant to be used within the diagnostic haematology laboratory, protecting equipment of selection of blood specimens, blood movie practise and marking, the foundations of guide and automatic blood counts and the evaluation of the morphological good points of blood cells. The working towards haematologist should still locate this booklet sufficiently accomplished to be a reference resource whereas, while, the trainee haematologist and biomedical scientist may still locate it an easy and useful bench manual.
Enables either the haematologist and laboratory scientist to spot blood mobile positive factors, from the commonest to the extra obscure
Provides crucial details on tools of assortment, blood movie training and marking, including the rules of handbook and automatic blood counts
Completely revised and up-to-date, incorporating a lot newly released info: now contains recommendation on additional assessments while a selected analysis is suspected
Four hundred top of the range photos to help with blood phone identification
- Highlights the aim and medical relevance of haematology laboratory checks all through
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Extra info for Blood Cells: A Practical Guide
6). Three simultaneous measurements are made on each cell: (i) impedance measurements with low-frequency electromagnetic current, dependent mainly on cell volume; (ii) conductivity Fig. 7 Histograms produced by a Coulter S Plus IV automated counter showing volume distribution of white cells, red cells and platelets. BCAC02 3/20/06 15:51 Page 37 Performing a blood count 37 (a) (b) (c) (d) Fig. 8 Printouts of Coulter STKS automated counter. (a) Scatter plot of white cell volume against discriminant function 1.
This method has been incorporated into directly reading haemoglobinometers, which are standardized to give the same result as a cyanmethaemoglobin method. Hb can be measured as haematin produced under alkaline conditions. The alkaline–haematin method measures carboxyhaemoglobin, sulphaemoglobin and methaemoglobin, although it does not adequately measure haemoglobin F or haemoglobin Bart’s, which are resistant to alkaline denaturation. An artificial standard is required. The acid–haematin method is less reliable and is not recommended.
The NCCLS classifies as a reticulocyte ‘any non-nucleated red cell containing two or more particles of bluestained material corresponding to ribosomal RNA’ . This definition is also accepted by the ICSH . The RNA, which is responsible for forming the reticulum following supravital staining, gives rise, on Romanowsky-stained films, to diffuse cytoplasmic basophilia. The combination of cytoplasmic basophilia with the acidophilia of haemoglobin produces staining characteristics known as polychromasia.